Our progress in the genetic manipulation of Clostridium cellulolyticum H10 is published on J. Microbiol. Methods

[2012.3.15] Our first paper with the title "Targeted gene engineering in Clostridium cellulolyticum H10 without methylation" is published on J. Microbiol. Methods now. The paper describes our progresses in the genetic manipulation of Clostridium cellulolyticum H10. A methylation-free genetic platform was developed with  high transformation efficiency, screening rate and stability.The mutant H10DmspI lost MspI activity and accepted unmethylated DNA efficiently, which is capable of heterologous gene expression and serial gene inactivation. Further, an anaerobic green fluorescent reporter system was constructed in H10DmspI. This genetic platform of C. cellulolyticum H10 will facilitate the metabolic engineering of C. cellulolyticum aiming at faster cellulose degradation and higher biofuel production at the molecular level.

The first author Gu-zhen Cui is a Ph.D. candidate student. The Metabolomics Group leader Prof. Dr. Qiu Cui and the Assistant Professor Dr. Ya-Jun Liu in the group are corresponding authors. This work was supported by One-hundred-Talented-People program (KSCX2-YW-G-066) from Chinese Academy of Sciences and National Basic Research Program of China (973-project, Grant No. 2011CB707404).

NCBI PubMed

Gu-zhen Cui, Wei Hong, Jie Zhang, Wen-li Li, Yingang Feng, Ya-jun Liu*, and Qiu Cui* (2012) Targeted gene engineering in Clostridium cellulolyticum H10 without methylation. J. Microbiol. Methods 89, 201-208. Full Text Link (Publisher website)