Research progress: Achievements in improving the production of polyunsaturated fatty acids in Aurantiochytrium by genetic manipulation

[2016.8.20] Aurantiochytrium sp. is a highly promising polyunsaturated fatty acid (PUFA) resource due to its high docosahexaenoic acid (DHA) production. The reducing agent NADPH is essential for the production of PUFA and other fatty acids, and glucose-6-phosphate dehydrogenase (G6PDH) is one of the most important enzymes for NADPH regeneration in Aurantiochytrium. To investigate whether PUFA production could be enhanced by stimulating G6PDH, a recombinant strain, termed SD116::G6PDH, was constructed by overexpressing G6PDH in Aurantiochytrium sp. SD116. As a result, G6PDH overexpression changed the fatty acid profile and enhanced the proportion of PUFA in lipids by 11%. These results confirmed the positive effect of G6PDH activity on PUFA production and suggested that the manipulation of NADPH production is a promising strategy for increasing PUFA production in Aurantiochytrium.
The results have been published in Algal Research on Aug. 20, 2016. The research was financially supported by the 863 Program from Ministry of Science and Technology of China, the National Natural Science Foundation of China and Qingdao Science and Technology Development Plan.

See the paper:
Gu-Zhen Cui, Zengxin Ma, Ya-Jun Liu, Yingang Feng, Zhijie Sun, Yurong Cheng, Xiaojin Song*, Qiu Cui (2016) Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116. Algal Res. 19, 138-145. [Full text (Publisher website)]